Identification and validation of Aeluropus littoralis reference genes for Quantitative Real-Time PCR Normalization
نویسندگان
چکیده
منابع مشابه
Validation of Reference Genes for Real Time PCR Normalization in Milk Somatic Cells of Holstein Dairy Cattle
Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...
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We have utilized an in vitro transcribed 3' mRNA fragment of the plant gene ribulose bisphosphate carboxylase (RuBisCO) as an exogenous standard for normalization of quantitative PCR data. Both K562 cells and primary erythroid CD34+ progenitor cells were treated with sodium butyrate and changes in gamma-globin mRNA levels were assayed using a previously published TaqMan probe and primer set, wh...
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ژورنال
عنوان ژورنال: Journal of Biological Research-Thessaloniki
سال: 2016
ISSN: 2241-5793
DOI: 10.1186/s40709-016-0053-8